KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media
| Autor: | Masafumi Katayama, Manabu Onuma, Tomokazu Fukuda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Journal of Poultry Science, Vol 58, Iss 4, Pp 270-279 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1346-7395 1349-0486 |
| DOI: | 10.2141/jpsa.0200085 |
| Popis: | Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|