Modification of an Automated Liquid-Handling System for Reagent-Jet, Nanoliter-Level Dispensing
| Autor: | J. Steven Hicks, Brent W. Harker, Kenneth L. Beattie, Mitchel J. Doktycz |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 30, Iss 4, Pp 878-885 (2001) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/01304rr05 |
| Popis: | Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton® MICROLAB® 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented. |
| Databáze: | Directory of Open Access Journals |
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