Use of Human Intestinal Enteroids to Detect Human Norovirus Infectivity

Autor:	Martin Chi-Wai Chan, Sarah K.C. Cheung, Kirran N. Mohammad, Jenny C.M. Chan, Mary K. Estes, Paul K.S. Chan
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Cycle threshold Ct cutoff enteroids infectivity human norovirus rRT-PCR Medicine Infectious and parasitic diseases RC109-216
Zdroj:	Emerging Infectious Diseases, Vol 25, Iss 9, Pp 1730-1735 (2019)
Druh dokumentu:	article
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid2509.190205
Popis:	Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/220a35accd554179b62ab1ccd347b35a Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF