NAT2 gene polymorphisms and plasma isoniazid concentration in Vietnamese tuberculosis patients
| Autor: | Anh Tuan Le, Nhung Hong Thi Pham, Long Doan Dinh, Luyen Thi Le, Thom Thi Vu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Walailak Journal of Science and Technology, Vol 18, Iss 1 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1686-3933 2228-835X |
| Popis: | Background: Isoniazid (INH) is one of the most common drug for tuberculosis (TB) treatment and INH acetylation catalysed by non-inducible hepatic enzyme arylamine N-acetyltransferase type 2 (NAT2). The isoniazid acetylation rates, which depends on NAT2 genotypes, is constant in an individual but can changes between patients. Phenotypic groups can be classified based on the genotype: slow, intermediate and rapid acetylators. This study was performed to identify the relation between NAT2 gene polymorphisms and plasma INH concentrations among the different genotypes of Vietnamese tuberculosis patients. Methods: Blood samples of 136 adult TB patients treated with INH were collected and genotyped for NAT2 gene polymorphisms using Sanger sequencing. Two-hour post-dosing INH plasma concentrations were determined by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). Results: Among the 136 patients genotyped, there were 43 (31.62%), 58 (42.65%) and 35 (25.74%) of slow, intermediate and rapid acetylation phenotypes with two-hour post dosing INH plasma concentration of 3.4, 2.7 and 2.2 μg/ml, respectively. The differences in INH concentrations among the three genotypes were significant (P |
| Databáze: | Directory of Open Access Journals |
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