A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

Autor: Edgar García-Fortea, Agustín Lluch-Ruiz, Benito José Pineda-Chaza, Ana García-Pérez, Juan Pablo Bracho-Gil, Mariola Plazas, Pietro Gramazio, Santiago Vilanova, Vicente Moreno, Jaime Prohens
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Plant Biology, Vol 20, Iss 1, Pp 1-16 (2020)
Druh dokumentu: article
ISSN: 1471-2229
DOI: 10.1186/s12870-019-2215-y
Popis: Abstract Background Efficient organogenesis induction in eggplant (Solanum melongena L.) is required for multiple in vitro culture applications. In this work, we aimed at developing a universal protocol for efficient in vitro regeneration of eggplant mainly based on the use of zeatin riboside (ZR). We evaluated the effect of seven combinations of ZR with indoleacetic acid (IAA) for organogenic regeneration in five genetically diverse S. melongena and one S. insanum L. accessions using two photoperiod conditions. In addition, the effect of six different concentrations of indolebutyric acid (IBA) in order to promote rooting was assessed to facilitate subsequent acclimatization of plants. The ploidy level of regenerated plants was studied. Results In a first experiment with accessions MEL1 and MEL3, significant (p
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje