Differential Gene Expression in Cell Types of the Human Skeletal Muscle: A Bioinformatics-Based Meta-Review
| Autor: | Kyung-Wan Baek, So-Jeong Kim, Ji-Seok Kim, Sun-Ok Kwon |
|---|---|
| Jazyk: | korejština |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | 운동과학, Vol 30, Iss 4, Pp 444-452 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1226-1726 2384-0544 |
| DOI: | 10.15857/ksep.2021.00577 |
| Popis: | PURPOSE This study evaluates the differences in the expression of genes frequently analyzed in the field of exercise science between the skeletal muscle tissue and various cell types that comprise the skeletal muscle tissue. METHODS We summarized the genes and proteins expressed in the skeletal muscle that were published in “Exercise Science” journal from 2015 to present. Thereafter, we selected 15 genes and proteins that were the most analyzed genes and proteins in the skeletal muscle. These genes and proteins were horizontally compared for expression differences in skeletal muscle components and cultured cells based on NCBI Gene Expression Omnibus DataSets. RESULTS The most analyzed genes (encoding analyzed proteins) in skeletal muscle tissues in “Exercise Science” were PPARGC1A, PPARD, MTOR, MAP1LC3A, MAP1LC3B, PRKAA1, AKT1, SLC2A4, MAPK1, COX4I1, MAPK14, MEF2A, MAPK8, RPS6KB1, and SOD1. Among them, PPARGC1A, AKT1, SLC2A4, MAPK1, and COX4I1 were specifically expressed in the skeletal muscle. However, expression of other genes was found to be significantly affected in other cell types of the skeletal muscle tissue. CONCLUSIONS Genes such as PPARGC1A, which are specifically expressed in the skeletal muscle, may be analyzed without pretreating (such as perfusion) the skeletal muscle tissue. However, expression of other genes may depend on the skeletal muscle cell type. Thus, in such instances, pretreatment, such as perfusion and isolation, should be considered. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|