A Terbium Sensitized Luminescence Method for the Assay of Flubiprofen in Pharmaceutical Formulations
| Autor: | Salma M.Z. Al-Kindy, Arwa Al-Ghazali, Fakhr Eldin O. Suliman, Haidar A.J. Al-Lawati |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Sultan Qaboos University Journal for Science, Vol 19, Iss 1, Pp 54-63 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1027-524X 2414-536X |
| DOI: | 10.24200/squjs.vol19iss1pp54-63 |
| Popis: | A sensitive time-resolved luminescence method for the determination of flubiprofen (FLP) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of terbium (Tb3+ ) by the formation of a ternary complex with FLP in the presence of 4,7 diphenyl 1,10 phenanthroline (DPP) as co-ligand, and Tween-20 as surfactant. The signal for Tb-FLP-DPP was monitored at λex = 285 nm and λem = 552 nm. Optimum conditions for the formation of the complex in an aqueous system were TRIS buffer, pH 8.0, DPP (2.5Å~10−7 M), Tween-20 (0.30%) and 4Å~10-5 mol L-1 of Tb3+ which allowed the determination of 20–1000 ng mL-1 of FLP with a limit of detection (LOD) of 10 ng mL-1 . The relative standard deviations of the method ranged between 0.6 and 1.4% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assays of FLP in pharmaceutical formulations and spiked tap water samples with average recoveries of 87% – 95%. |
| Databáze: | Directory of Open Access Journals |
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