| Popis: |
Objective To study the feasibility and effectiveness of quantitative evaluation of retinal function in blind patients with retinitis pigmentosa (RP) by chromatic pupillometry. Methods A total of 46 blind RP patients (81 eyes) who met the legal blindness standard admitted in our department from January 2022 to December 2022 were recruited in this study.According to their best-corrected visual acuity, they were divided into the no form perception (NFP) group, faint form perception (FFP) group, and form perception (FP) group.Another 18 healthy volunteers (18 eyes) were enrolled as controls.The subjects received routine ophthalmic examinations such as color fundus photography, optical coherence tomography (OCT), fundus autofluorescence (FAF), and flash visual evoked potential (FVEP).The thickness of the outer nuclear layer of OCT, vertical diameter of the FAF high fluorescence area, and amplitude of FVEP P2 were collected.Then chromatic pupillometry was performed.The light stimulation mode of low-intensity blue light was conducted to activate rods, and of high-intensity red light to activate cones.The evaluation indexes included transient pupil construction amplitude (TPCA), latency, maximal contraction velocity (MCV), and contraction duration. Results Under the mode of low-intensity blue light stimulation, more than half of the blind RP patients had rods pupil light reaction disappeared.Under the mode of high-intensity red light stimulation, the blind RP patients though retained pupil response of cone cells to light mostly but the response was significantly weakened.Compared with the control group, the TPCA and MCV of the cones'pupillary responses were significantly lower (P < 0.05), and the contraction latency was obviously higher (P < 0.05) in all RP blind groups.The NFP group had significantly lower TPCA and MCV and higher contraction latency when compared with the FP group (both P < 0.05).Positively correlations were observed in the TPCA and MCV of the cones'pupillary responses with the thickness of the outer nuclear layer of the retina, the vertical diameter of autofluorescence, and the amplitude of the FVEP P2 wave, respectively (P < 0.05).The latency of contraction was significantly negatively correlated with the thickness of the outer nuclear layer of the retina and the amplitude of FVEP P2 wave (P < 0.05). Conclusion Chromatic pupillometry can quantitatively distinguish the difference in retinal function between blind RP patients with and without formal perceptual vision.The changes in chromatic pupillometric results are consistent with the changes in retinal morphology and function. |
