WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
| Autor: | Liang Huang, Jinfan Shao, Xijuan Xu, Weiwen Hong, Wenfeng Yu, Shuang Zheng, Xiaogang Ge |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Cell Adhesion & Migration, Vol 17, Iss 1, Pp 1-13 (2023) |
| Druh dokumentu: | article |
| ISSN: | 19336918 1933-6926 1933-6918 |
| DOI: | 10.1080/19336918.2023.2180196 |
| Popis: | ABSTRACTOur study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expression of WTAP, FLNA and autophagy-related proteins in cells. Our results confirmed the up-regulation of WTAP in colon cancer and its promoting effect on proliferation and inhibiting effect on apoptosis. FLNA was the downstream gene of WTAP and WTAP-regulated m6A modification led to post-transcriptional repression of FLNA. The rescue experiments showed that WTAP/FLNA could inhibit autophagy. WTAP-mediated m6A modification was confirmed to be crucial in colon cancer development, providing new insights into colon cancer therapy. |
| Databáze: | Directory of Open Access Journals |
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