Enhancing multi-spot structured illumination microscopy with fluorescence difference
| Autor: | Edward N. Ward, Frida H. Torkelsen, Robert Pal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Royal Society Open Science, Vol 5, Iss 3 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2054-5703 95478671 |
| DOI: | 10.1098/rsos.171336 |
| Popis: | Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|