| Popis: |
Singlet lenses are free from precise assembling, aligning, and testing, which are helpful for the development of portable and low-cost microscopes. However, balancing the spectrum dispersion or chromatic aberrations using a singlet lens made of one material is difficult. Here, a novel method combining singlet lens microscopy and computational imaging, which is based on deep learning image-style-transfer algorithms, is proposed to overcome this problem in clinical pathological slide microscopy. In this manuscript, a singlet aspheric lens is used, which has a high cut-off frequency and linear signal properties. Enhanced by a trained deep learning network, it is easy to transfer the monochromatic gray-scale microscopy picture to a colorful microscopy picture, with only one single-shot recording by a monochromatic CMOS image sensor. By experiments, data analysis, and discussions, it is proved that our proposed virtual colorization microscope imaging method is effective for H&E stained tumor tissue slides in singlet microscopy. It is believable that the computational virtual colorization method for singlet microscopes would promote the low-cost and portable singlet microscopy development in medical pathological label staining observing (e.g., H&E staining, Gram staining, and fluorescent labeling) biomedical research. |
