Conversion of Adipose-Derived Stem Cells into Sweat Gland-Like Cells: An In Vitro Phenotypic Study
| Autor: | Shuai Qiang, Xiao-Meng Wang, Cheng-Kun Zhang, Ying Liu, Zhi-Bo Xiao, Qiang Li, Feng-Yong Li, Yu Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | International Journal of Dermatology and Venerology, Vol 3, Iss 3, Pp 166-170 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2096-5540 2641-8746 00000000 |
| DOI: | 10.1097/JD9.0000000000000081 |
| Popis: | Abstract. Objective:. This study was performed to explore the conversion of adipose-derived stem cells (ADSCs) into sweat gland-like cells for the purpose of sweat gland regeneration. Methods:. ADSCs and human sweat gland (hSG) cells were isolated, cultured, and identified. The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells. Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers. Results:. The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers (cytokeratins 7, 14, and 18). Conclusion:. These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation. ADSCs may serve as a potential source of cells for sweat gland regeneration. |
| Databáze: | Directory of Open Access Journals |
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