| Autor: |
Anna Kodisch, Michael Oberforster, Armin Raditschnig, Bernd Rodemann, Anna Tratwal, Jakub Danielewicz, Marek Korbas, Brigitta Schmiedchen, Jakob Eifler, Andres Gordillo, Dörthe Siekmann, Franz Joachim Fromme, Frederik N. Wuppermann, Franz Wieser, Elisabeth Zechner, Małgorzata Niewińska, Thomas Miedaner |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Toxins, Vol 12, Iss 11, p 676 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2072-6651 |
| DOI: |
10.3390/toxins12110676 |
| Popis: |
Ergot caused by Claviceps purpurea is a problem for food and feed security in rye due to the occurrence of toxic ergot alkaloids (EAs). For grain elevators and breeders, a quick, easy-to-handle, and cheap screening assay would have a high economic impact. The study was performed to reveal (1) the covariation of ergot severity (= percentage of sclerotia in harvested grain) and the content of 12 EAs determined by high performance liquid chromatography (HPLC) and (2) the covariation between these traits and results of one commercial enzyme linked immunosorbent assays (ELISA). In total, 372 winter rye samples consisting of a diverse set of genotypes, locations from Germany, Austria, and Poland over two years, and three isolates were analyzed. Ergocornine and α-ergocryptine were detected as major EAs. Ergocristinine occurred as a minor component. Claviceps isolates from different countries showed a similar EA spectrum, but different quantities of individual EAs. A moderate, positive covariation between ergot severity and EA content determined by HPLC was observed across two years (r = 0.53, p < 0.01), but large deviation from the regression was detected. ELISA values did neither correlate with the HPLC results nor with ergot severity. In conclusion, a reliable prediction of the EA content based on ergot severity is, at present, not possible. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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