Chimeric Peptides from Californiconus californicus and Heterodontus francisci with Antigen-Binding Capacity: A Conotoxin Scaffold to Create Non-Natural Antibodies (NoNaBodies)

Autor: Salvador Dueñas, Teresa Escalante, Jahaziel Gasperin-Bulbarela, Johanna Bernáldez-Sarabia, Karla Cervantes-Luévano, Samanta Jiménez, Noemí Sánchez-Campos, Olivia Cabanillas-Bernal, Blanca J. Valdovinos-Navarro, Angélica Álvarez-Lee, Marco A. De León-Nava, Alexei F. Licea-Navarro
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Toxins, Vol 15, Iss 4, p 269 (2023)
Druh dokumentu: article
ISSN: 2072-6651
DOI: 10.3390/toxins15040269
Popis: Research into various proteins capable of blocking metabolic pathways has improved the detection and treatment of multiple pathologies associated with the malfunction and overexpression of different metabolites. However, antigen-binding proteins have limitations. To overcome the disadvantages of the available antigen-binding proteins, the present investigation aims to provide chimeric antigen-binding peptides by binding a complementarity-determining region 3 (CDR3) of variable domains of new antigen receptors (VNARs) with a conotoxin. Six non-natural antibodies (NoNaBodies) were obtained from the complexes of conotoxin cal14.1a with six CDR3s from the VNARs of Heterodontus francisci and two NoNaBodies from the VNARs of other shark species. The peptides cal_P98Y vs. vascular endothelial growth factor 165 (VEGF165), cal_T10 vs. transforming growth factor beta (TGF-β), and cal_CV043 vs. carcinoembryonic antigen (CEA) showed in-silico and in vitro recognition capacity. Likewise, cal_P98Y and cal_CV043 demonstrated the capacity to neutralize the antigens for which they were designed.
Databáze: Directory of Open Access Journals
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