Experimental modeling of aerosols produced by microorganisms in working area air as risk factor exerting hazardous impacts on health of workers employed at biotechnological production
| Autor: | N.V. Dudchik, V.A. Filonyuk, V.V. Shevlyakov, S.I. Sychik, O.E. Nezhvinskaya |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Analiz Riska Zdorovʹû, Iss 3, Pp 127-134 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2308-1155 2308-1163 |
| DOI: | 10.21668/health.risk/2017.3.15.eng |
| Popis: | Scientific foundation and practices in the sphere of hygienic and ecological standardization concerning biological factors of the environment have a number of peculiarities and are methodically less developed than chemical factors standardization. Efficient industrial control over maximum permissible concentrations of standardized microorganisms-producers in working area air is based on validated instrumental techniques of quantitative assessment. Our goal was to create experimental models for microorganisms-producers' aerosols of a multi-component microbe specimen in working area air as a risk factor causing impacts on health of workers employed at biotechnological production; another task was to work out a procedure for measuring Pseudomonas aurantiaca B-162/255.17concentration and cells and spores of Bacillus sp. BB58-3 strain in working area air. We gave grounds for a technology aimed at quantitative determination of microorganisms-producers in working area air in a modeling experiment; it was based on conventional stages and tech-niques accepted in microbiological practices, namely air samples taking via aspiration technique allowing for a volume taken; cultivation under conditions which are optimal for examined microorganisms-producers in a nutrient medium with reduced composition; calculation of evolved colonies with specific morphological features; morphologic identification of microorganisms and colonies; calculation of microorganisms' quantity on dishes with recalculation per 1 m3 of air. Bas-ing on the detected regular concentration dependences of microbe contamination dynamics in air we worked out a proce-dure for quantitative determination of microorganisms-producers; we also performed metrological estimate of opera-tional properties for assessing microorganisms-producers of a multi-component microbe specimen as a risk factor caus-ing hazardous impacts on health of workers employed at biotechnological production. We validated our measuring procedure in conformity with the requirements set forth by ISO. |
| Databáze: | Directory of Open Access Journals |
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