Genetic traceability of Staphylococcus aureus strains isolated from primiparous dairy cows mastitis, humans and environment in the Northeast region of Brazil
| Autor: | Amanda Thaís Ferreira Silva, José Givanildo da Silva, Breno Bezerra Aragão, Núbia Michelle Vieira da Silva, Priscylla Carvalho Vasconcelos, Celso José Bruno de Oliveira, Rinaldo Aparecido Mota |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 51, Iss 4 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20200679 |
| Popis: | ABSTRACT: This research aimed to investigate the genotypic relatedness of 18 Staphylococcus aureus strains isolated from intramammary infections in primiparous cows and extramammary sites on five dairy herds by rep-PCR using RW3A primers, and by PFGE using the endonuclease SmaI. The isolates were also evaluated in vitro for the susceptibility against beta-lactam antimicrobials drugs (penicillin and oxacillin), considering that beta-lactams are frequently used for treating staphylococcal intrammamary infections. The rep-PCR typing was highly discriminatory (D value= 0.9804) and a total of 15 patterns were detected. The PFGE method was also highly discriminatory (D value= 0.9667) and a total of 13 patterns were observed. A total of 15 out of 18 (83%) isolates were resistant to penicillin and one out of 18 (6%) to oxacillin. In conclusion, these findings confirmed the occurrence of a high genetic diversity of S. aureus strains at the herds and the presence of clonally-related strains only at the same herd, emphasizing a variety of genotypic profiles among the isolates. |
| Databáze: | Directory of Open Access Journals |
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