(GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso

Autor: Boukaré Kaboré, Ganamé Abasse Ouédraogo, Hama Cissé, Henri S. Ouédraogo, Emmanuel Sampo, Koudbi Jacob Zongo, Boukaré Zeba, Yves Traoré, Olivier Gnankiné, Idrissa Sanou, Aly Savadogo
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: BMC Microbiology, Vol 22, Iss 1, Pp 1-8 (2022)
Druh dokumentu: article
ISSN: 1471-2180
DOI: 10.1186/s12866-022-02537-7
Popis: Abstract Background Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR. Results Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). Conclusion The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones.
Databáze: Directory of Open Access Journals
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