Multi‐Scale Label‐Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two‐Photon Microscopy

Autor: Shuaibin Chang, Jiarui Yang, Anna Novoseltseva, Ayman Abdelhakeem, Mackenzie Hyman, Xinlei Fu, Chenglin Li, Shih‐Chi Chen, Jean C. Augustinack, Caroline Magnain, Bruce Fischl, Ann C. Mckee, David A. Boas, Ichun Anderson Chen, Hui Wang
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Advanced Science, Vol 10, Iss 35, Pp n/a-n/a (2023)
Druh dokumentu: article
ISSN: 2198-3844
20230338
DOI: 10.1002/advs.202303381
Popis: Abstract The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation‐free reconstruction. Here, the authors describe an integrated serial sectioning polarization‐sensitive optical coherence tomography (PSOCT) and two photon microscopy (2PM) system to provide label‐free multi‐contrast imaging of intact brain structures, including scattering, birefringence, and autofluorescence of human brain tissue. The authors demonstrate high‐throughput reconstruction of 4 × 4 × 2cm3 sample blocks and simple registration between PSOCT and 2PM images that enable comprehensive analysis of myelin content, vascular structure, and cellular information. The high‐resolution 2PM images provide microscopic validation and enrichment of the cellular information provided by the PSOCT optical properties on the same sample, revealing the densely packed fibers, capillaries, and lipofuscin‐filled cell bodies in the cortex and white matter. It is shown that the imaging system enables quantitative characterization of various pathological features in aging process, including myelin degradation, lipofuscin accumulation, and microvascular changes, which opens up numerous opportunities in the study of neurodegenerative diseases in the future.
Databáze: Directory of Open Access Journals
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