| Autor: |
Constance Onfray, Simon Chevolleau, Eva Moinard, Océane Girard, Kasturi Mahadik, Ryan Allsop, Grigorios Georgolopoulos, Régis Lavigne, Ophélie Renoult, Irene Aksoy, Elsa Lemaitre, Philippe Hulin, Jean-François Ouimette, Thomas Fréour, Claire Pecqueur, Charles Pineau, Vincent Pasque, Claire Rougeulle, Laurent David |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Cell Reports, Vol 43, Iss 5, Pp 114232- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2024.114232 |
| Popis: |
Summary: The advent of novel 2D and 3D models for human development, including trophoblast stem cells and blastoids, has expanded opportunities for investigating early developmental events, gradually illuminating the enigmatic realm of human development. While these innovations have ushered in new prospects, it has become essential to establish well-defined benchmarks for the cell sources of these models. We aimed to propose a comprehensive characterization of pluripotent and trophoblastic stem cell models by employing a combination of transcriptomic, proteomic, epigenetic, and metabolic approaches. Our findings reveal that extended pluripotent stem cells share many characteristics with primed pluripotent stem cells, with the exception of metabolic activity. Furthermore, our research demonstrates that DNA hypomethylation and high metabolic activity define trophoblast stem cells. These results underscore the necessity of considering multiple hallmarks of pluripotency rather than relying on a single criterion. Multiplying hallmarks alleviate stage-matching bias. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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