Identification and functional characterization of lncRNAs involved in human monocyte-to-macrophage differentiation
| Autor: | Christy Montano, Sergio Covarrubias, Eric Malekos, Sol Katzman, Susan Carpenter |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | RNA Biology, Vol 21, Iss 1, Pp 39-51 (2024) |
| Druh dokumentu: | article |
| ISSN: | 15476286 1555-8584 1547-6286 |
| DOI: | 10.1080/15476286.2024.2417155 |
| Popis: | Although long noncoding RNAs (lncRNAs) constitute the majority of the human transcriptome, the functional roles of most remain elusive. While protein-coding genes in macrophage biology have been extensively studied, the contribution of lncRNAs in this context is poorly understood. Given the vast number of lncRNAs (>20,000), identifying candidates for functional characterization poses a significant challenge. Here, we present two complementary approaches to pinpoint and investigate lncRNAs involved in monocyte-to-macrophage differentiation: RNA-seq for functional inference and a high-throughput functional screen. These strategies enabled us to identify four lncRNA regulators of monocyte differentiation: lincRNA-JADE1, lincRNA-ANXA3, GATA2-AS1, and PPP2R5C-AS1. Preliminary insights suggest these lncRNAs may act in cis through neighbouring protein-coding genes, although their precise mechanisms remain to be elucidated. We further discuss the strengths and weaknesses of these methodologies, along with validation pipelines crucial for establishing lncRNA functionality. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|