CRISPR–Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis

Autor: GiWon Shin, Susan M. Grimes, HoJoon Lee, Billy T. Lau, Li C. Xia, Hanlee P. Ji
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/ncomms14291
Popis: Microsatellite genotyping is a common molecular method of for identification but current methods have restricted throughput. The authors demonstrate STR-Seq, which combines next generation sequencing with targetedin vitroCRISPR-Cas9 fragmentation to enable massively parallel analysis in the thousands.
Databáze: Directory of Open Access Journals