| Autor: |
Alana A. Borges, Maria V.O. Santos, Luiza B. Queiroz Neta, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira |
| Jazyk: |
English<br />Portuguese |
| Předmět: |
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| Zdroj: |
Pesquisa Veterinária Brasileira, Vol 38, Iss 9, Pp 1863-1868 |
| Druh dokumentu: |
article |
| ISSN: |
1678-5150 |
| DOI: |
10.1590/1678-5150-pvb-5471 |
| Popis: |
ABSTRACT: Oocyte in vitro maturation (IVM) is the first step of the in vitro reproductive technologies that enables mature oocytes to be generated ex vivo and after used for embryo production. In this sense, the establishment of culture environment, as oocyte incubation time, is essential for the success of the IVM. Therefore, the study was carried out to investigate the relationship between the meiotic potential and the IVM times of collared peccary oocytes, wild mammals of great commercial and ecological interest. Thus, ovaries were collected of females derived from captivity and transported to the laboratory within 1 hour of slaughtering. The oocytes derived from follicles (3-6mm in diameter) were recovered by aspirated and sliced. Good quality oocytes (evenly granulated cytoplasm with a least one layer of surrounding cumulus cells) were selected and subjected to culture in TCM 199 supplemented with 10µg/mL FSH, 10% FBS and 100µM cysteamine at 38.5°C, 5% CO2 and maximum humidity for 24 or 48 hours. After the incubation period, the nuclear status, the presence of first polar body and the expansion of cumulus cells of oocytes were assessed. The data obtained were analyzed by Fisher exact test (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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