| Autor: |
Sarah Tassinari, Edoardo D'Angelo, Federico Caicci, Cristina Grange, Jacopo Burrello, Matteo Fassan, Alessia Brossa, Riccardo Quoc Bao, Gaya Spolverato, Marco Agostini, Federica Collino, Benedetta Bussolati |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Extracellular Biology, Vol 3, Iss 3, Pp n/a-n/a (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2768-2811 |
| DOI: |
10.1002/jex2.144 |
| Popis: |
Abstract Cellular elements that infiltrate and surround tumours and pre‐metastatic tissues have a prominent role in tumour invasion and growth. The extracellular vesicles specifically entrapped and stored within the extracellular matrix (ECM‐EVs) may reflect the different populations of the tumour microenvironment and their change during tumour progression. However, their profile is at present unknown. To elucidate this aspect, we isolated and characterized EVs from decellularized surgical specimens of colorectal cancer and adjacent colon mucosa and analyzed their surface marker profile. ECM‐EVs in tumours and surrounding mucosa mainly expressed markers of lymphocytes, natural killer cells, antigen‐presenting cells, and platelets, as well as epithelial cells, representing a multicellular microenvironment. No difference in surface marker expression was observed between tumour and mucosa ECM‐EVs in stage II‐III tumours. At variance, in the colon mucosa adjacent to stage IV carcinomas, ECM‐EV profile showed a significantly increased level of immune, epithelial and platelet markers in comparison to the matrix of the corresponding tumour. The increase of EVs from immune cells and platelets was not observed in the mucosa adjacent to low‐stage tumours. In addition, CD25, a T‐lymphocyte marker, resulted specifically overexpressed by ECM‐EVs from stage IV carcinomas, possibly correlated with the pro‐tolerogenic environment found in the corresponding tumour tissue. These results outline the tissue microenvironmental profile of EVs in colorectal carcinoma‐derived ECM and unveil a profound change in the healthy mucosa adjacent to high‐stage tumours. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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