| Autor: |
Marta Iozzo, Francesco Vitali, Carolina Chiellini, Leandro Gammuto, Antonio Taddei, Amedeo Amedei, Renato Fani |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Bioscience-Landmark, Vol 27, Iss 11, p 305 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2768-6701 |
| DOI: |
10.31083/j.fbl2711305 |
| Popis: |
Background: Azurin, a bacterial cupredoxin firstly isolated from the bacterium Pseudomonas aeruginosa, is considered a potential alternative therapeutic tool against different types of cancer. Aims: In this work we have explored the relationship possibly existing between azurin and colorectal cancer (CRC), in light of the evidence that microbial imbalance can lead to CRC progression. Methodology/Results: To this aim, the presence of azurin coding gene in the DNA extracted from saliva, stool, and biopsy samples of 10 CRC patients and 10 healthy controls was evaluated by real-time PCR using primers specifically designed to target the azurin coding gene from different bacterial groups. The correlation of the previously obtained microbiota data with real-time PCR results evidenced a “preferential” enrichment of seven bacterial groups in some samples than in others, even though no statistical significance was detected between controls and CRC. The subset of azurin gene-harbouring bacterial groups was representative of the entire community. Conclusions: Despite the lack of statistical significance between healthy and diseased patients, HTS data analysis highlighted a kind of “preferential” enrichment of seven bacterial groups harbouring the azurin gene in some samples than in others. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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