Competition between kinesin-1 and myosin-V defines Drosophila posterior determination

Autor: Wen Lu, Margot Lakonishok, Rong Liu, Neil Billington, Ashley Rich, Michael Glotzer, James R Sellers, Vladimir I Gelfand
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: eLife, Vol 9 (2020)
Druh dokumentu: article
ISSN: 2050-084X
DOI: 10.7554/eLife.54216
Popis: Local accumulation of oskar (osk) mRNA in the Drosophila oocyte determines the posterior pole of the future embryo. Two major cytoskeletal components, microtubules and actin filaments, together with a microtubule motor, kinesin-1, and an actin motor, myosin-V, are essential for osk mRNA posterior localization. In this study, we use Staufen, an RNA-binding protein that colocalizes with osk mRNA, as a proxy for osk mRNA. We demonstrate that posterior localization of osk/Staufen is determined by competition between kinesin-1 and myosin-V. While kinesin-1 removes osk/Staufen from the cortex along microtubules, myosin-V anchors osk/Staufen at the cortex. Myosin-V wins over kinesin-1 at the posterior pole due to low microtubule density at this site, while kinesin-1 wins at anterior and lateral positions because they have high density of cortically-anchored microtubules. As a result, posterior determinants are removed from the anterior and lateral cortex but retained at the posterior pole. Thus, posterior determination of Drosophila oocytes is defined by kinesin-myosin competition, whose outcome is primarily determined by cortical microtubule density.
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