| Autor: |
Brian P Blackwood, Douglas R Wood, Carrie Y Yuan, Joseph D Nicolas, Anne Griffiths, Karen Mestan, Catherine J Hunter |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Journal of Neonatal Surgery, Vol 4, Iss 4 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2226-0439 |
| DOI: |
10.21699/jns.v4i4.282 |
| Popis: |
Background: Necrotizing Enterocolitis (NEC) affects 5-10% of NICU patients where initially patients may have only nonspecific clinical findings. A noninvasive tool for detection would aid in diagnosis. Increased urinary claudins have been associated with active adult inflammatory bowel disease. Methods: Institutional Review Board approval was obtained. Neonatal intestinal tissue samples were obtained from patients with and without NEC. Immunofluorescence analysis of claudin-2 was performed on the intestinal tissue. Thirty two urine samples were collected from 6 NICU patients. Proteins were extracted and urinary claudin-2 expression was measured using Western Blot Analysis. All sample concentrations were normalized to urinary creatinine. Differences were analyzed with ANOVA or Student’s T-test. Findings were correlated to the patient’s clinical status. Results: Neonatal intestinal immunofluorescence analysis revealed that claudin-2 is present in healthy intestinal epithelium and is decreased in NEC intestinal tissue (p=0.0001). Of the six patients evaluated, three patients had NEC. All 3 patients with NEC had spikes in urinary claudin-2 levels (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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