Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints
| Autor: | Savoldi-Barbosa Marcela, Sakamoto-Hojo Elza T., Takahashi Catarina S. |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 22, Iss 2, Pp 217-223 (1999) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| Popis: | Experiments with novobiocin (NB) post-treatment were performed to verify its effect on the frequencies of micronuclei (MN) and chromosomal aberrations (CA) induced by g-irradiation (0.75, 1.5 and 3.0 Gy) in human lymphocytes at G0-phase. The frequencies of MN significantly decreased by 44 and 50%, for the treatment with NB 50 µg/ml (30-min pulse) after radiation doses of 1.5 and 3.0 Gy, respectively. However, CA frequencies were not significantly affected. No significant effect on CA was observed when lymphocyte cultures were exposed to a single dose of 2.0 Gy at the G0-phase and posttreated with 25 µg/ml NB for three hours either immediately after irradiation (G0-phase) or after 24 h (S-phase). The significant suppressive effect of NB on MN frequencies supports the hypothesis that NB interaction with chromatin increases access to DNA repair enzymes. |
| Databáze: | Directory of Open Access Journals |
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