Methanolic Fractions of Ornithogalum cuspidatum Induce Apoptosis in PC-3 Prostate Cancer Cell Line and WEHI-164 Fibrosarcoma Cancer Cell Line
Autor: | Hamed Asadi, Mona Orangi, Dariush Shanehbandi, Zohreh Babaloo, Abbas Delazar, Leila Mohammadnejad, Fatemeh Zare Shahneh, Samira Valiyari, Behzad Baradaran |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: | |
Zdroj: | Advanced Pharmaceutical Bulletin, Vol 4, Iss suppl 1, Pp 455-458 (2014) |
Druh dokumentu: | article |
ISSN: | 2228-5881 2251-7308 |
DOI: | 10.5681/apb.2014.067 |
Popis: | Purpose: The present study, was aimed to assess the cytotoxic effects of Ornithogalum cuspidatum methanolic fractions on PC-3, prostate cancer cells and WEHI-164, Fibrosarcoma cells. Methods: Methanolic fractions of O. cuspidatum were prepared using solid phase extraction and the cells were treated with different concentrations for 12 and 24 hours. Cytotoxicity and cell viability were measured by MTT assay. ELISA was also employed to assess the histone-associated DNA fragments and the involvement of apoptotic mechanisms. Results: 10 and 20% fractions had not significant cytotoxic effects (p>0.05) but other fractions exerted growth inhibition on both cancer cell lines (p |
Databáze: | Directory of Open Access Journals |
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