Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies

Autor:	Elaine Machado Martinez, Jorge Antonio Santos Correia, Erika Verissimo Villela, Antonio Nascimento Duarte, Luiz Fernando Ferreira, Alexandre Ribeiro Bello
Jazyk:	angličtina
Rok vydání:	2003
Předmět:	random amplified polymorphic DNA analysis Trichuris trichiura paleoparasitology Microbiology QR1-502 Infectious and parasitic diseases RC109-216
Zdroj:	Memorias do Instituto Oswaldo Cruz, Vol 98, Pp 59-62 (2003)
Druh dokumentu:	article
ISSN:	0074-0276 1678-8060
DOI:	10.1590/S0074-02762003000900011
Popis:	Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/1e8f3a9b845843e7b98b6a3db73fc6c4 Zobrazit plný text záznamu View record in DOAJ