| Autor: |
Haoyang Li, Quan Lu, Zhong Wang, Wenbo Zhang, Yu Wu, Yandong Sun, Yue Hu, Lehui Xiao, Dongping Zhong, Suhui Deng, Shangguo Hou |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Communications Engineering, Vol 3, Iss 1, Pp 1-10 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2731-3395 |
| DOI: |
10.1038/s44172-024-00320-2 |
| Popis: |
Abstract Understanding biological activities in cells or deep tissues requires high-speed three-dimensional (3D) imaging. Substantial progress has been made with the emergence of 3D random-access microscopy. However, current solutions for fast 3D random-access imaging remain complex and costly. Herein we propose a simple, cost-effective, and fast 3D random-access confocal microscopy with remote focusing system. Our system shows isotropic response times across the x, y, and z axes, with a 34-fold improvement in axial response time over traditional piezo stages. We demonstrate its volumetric imaging performance with fluorescent particles and live cells. Furthermore, we validate the 3D random-access imaging capability of this system by continuously monitoring the signals in three different planes, showing a refresh rate of 500 Hz on two different positions in 3D. The simplicity, versatility, and affordability of our system promise widespread applications in research and industry. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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