| Autor: |
Marcelo Luiz Lima Brandão, Davi de Oliveira Almeida, Victor Augustus Marin, Marize Pereira Miagostovich |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia, Vol 2, Iss 3 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2317-269X |
| Popis: |
Phosphate saline buffer (PBS) and glycine buffer (GB) were evaluated as elution buffers in an adsorption-elution method using a negatively charged membrane associated with quantitative polymerase chain reaction (qPCR) and seminested PCR for detection of Norovirus genogroup II (NoV GII) from lettuce. In this methodology, PP7 bacterio-phage was used as a virus sample for process control. The qPCR showed more sensitivity than semi-nested PCR for NoV GII detection. The recovery efficiency, using PBS and GB, ranged from 24.72 to 60.78% and 19.48 to 137.26% for NoV GII, and from 0.01 to 0.15% and 0.13 to 6.04% for PP7 bacteriophage, respectively. Elution with GB was more effi-cient for PP7 bacteriophage recovery (p = 0.03), but no difference was seen for NoV GII (p = 0.57). The GB performed better than PBS as an eluent solution and can be consid-ered a methodological improvement. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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