Comparative Analysis of Policosanols Related to Growth Times from the Seedlings of Various Korean Oat (Avena sativa L.) Cultivars and Screening for Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Activation

Autor: Han-Gyeol Lee, So-Yeun Woo, Hyung-Jae Ahn, Ji-Yeong Yang, Mi-Ja Lee, Hyun-Young Kim, Seung-Yeob Song, Jin-Hwan Lee, Woo-Duck Seo
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Plants, Vol 11, Iss 14, p 1844 (2022)
Druh dokumentu: article
ISSN: 2223-7747
DOI: 10.3390/plants11141844
Popis: The objectives of this research were to evaluate the policosanol profiles and adenosine-5′-monophosphate-activated protein kinase (AMPK) properties in the seedlings of Korean oat (Avena sativa L.) cultivars at different growth times. Nine policosanols in the silylated hexane extracts were detected using GC-MS and their contents showed considerable differences; specifically, hexacosanol (6) exhibited the highest composition, constituting 88–91% of the total average content. Moreover, the average hexacosanol (6) contents showed remarkable variations of 337.8 (5 days) → 416.8 (7 days) → 458.9 (9 days) → 490.0 (11 days) → 479.2 (13 days) → 427.0 mg/100 g (15 days). The seedlings collected at 11 days showed the highest average policosanol content (541.7 mg/100 g), with the lowest content being 383.4 mg/100 g after 5 days. Interestingly, policosanols from oat seedlings grown for 11 days induced the most prevalent phenotype of AMPK activation in HepG2 cells, indicating that policosanols are an excellent AMPK activator.
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