Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry
| Autor: | Rottmann Jonas, Schierbaum Nicolas, Netaev Alexander, Ligges Manuel, Seidl Karsten |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Current Directions in Biomedical Engineering, Vol 9, Iss 1, Pp 363-366 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2364-5504 2023-1091 |
| DOI: | 10.1515/cdbme-2023-1091 |
| Popis: | Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the application of this method. We summarize the requirements for a suited detector for time resolved flow cytometry (TRFC) and present a new developed SPAD array detector with 100 pixels and 340 ns time between measurement windows, allowing a high-throughput of up to 60,000 cells/s. The functionality of the detector is shown with the measurement of a 22.2 ns laser pulse, making it applicable for an improved TRFC. |
| Databáze: | Directory of Open Access Journals |
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