| Autor: |
Steffane Q. Nascimento, Rodrigo M. Iost, Thiago C. Oliveira, Rafael N. Colombo, Luana C.I. Faria, Thiago Bertaglia, Jéssica C. Pacheco, Mona N. Oliveira, Erika R. Manuli, Geovana M. Pereira, Ester C. Sabino, Frank N. Crespilho |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Biosensors and Bioelectronics: X, Vol 18, Iss , Pp 100472- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2590-1370 |
| DOI: |
10.1016/j.biosx.2024.100472 |
| Popis: |
The ongoing COVID-19 pandemic continues to have a significant impact on our daily lives, necessitating the rapid development of early diagnostic tools to mitigate the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks. In this context, biosensor technology has emerged as a highly promising strategy to address the challenges of low sensitivity, specificity, and high cost associated with clinical diagnosis. In this study, we present a novel and cost-effective approach for the rapid detection of SARS-CoV-2 using miniaturized flexible carbon fiber (FCF) electrodes that are modified with immunoglobulin G (IgG). Our strategy take advantage of on the antigen-antibody interaction (IgG-SARS-CoV-2) and leverages the surface chemistry characteristics of FCF to achieve signal amplification. Under standard conditions, we achieved a remarkable detection limit of 0.16 pg mmL−1 for the SARS-CoV-2 RBD protein. Additionally, when analyzing human saliva samples, our biosensing approach demonstrated good agreement with RT-PCR results, specifically for patients who tested positive for SARS-CoV-2. The sensitivity, selectivity, and accuracy of our approach were approximately 93.3%. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
