Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm

Autor:	Md. Mazharul Islam, Takashi Umehara, Natsumi Tsujita, Masayuki Shimada
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	fatty acid transporter frozen sperm mitochondria oxygen consumption mitochondrial β‐oxidation sperm motility pattern Diseases of the endocrine glands. Clinical endocrinology RC648-665 Reproduction QH471-489
Zdroj:	Reproductive Medicine and Biology, Vol 20, Iss 3, Pp 289-298 (2021)
Druh dokumentu:	article
ISSN:	1447-0578 1445-5781
DOI:	10.1002/rmb2.12381
Popis:	Abstract Purpose The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Methods Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility was analyzed. The kinetic changes in FA levels in sperm were detected using gas chromatography‐mass spectrometry. The mitochondrial activity of sperm thawed in media containing LM or FAs was analyzed based on the fluorescence intensity of JC‐1 staining and the oxygen consumption rate. FA transporters were observed using whole‐mounted immunofluorescence. Results Sperm linear motility was significantly (P
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