SMART-LAMP: A Smartphone-Operated Handheld Device for Real-Time Colorimetric Point-of-Care Diagnosis of Infectious Diseases via Loop-Mediated Isothermal Amplification
| Autor: | Juan García-Bernalt Diego, Pedro Fernández-Soto, Sergio Márquez-Sánchez, Daniel Santos Santos, Begoña Febrer-Sendra, Beatriz Crego-Vicente, Juan Luis Muñoz-Bellido, Moncef Belhassen-García, Juan M. Corchado Rodríguez, Antonio Muro |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Biosensors, Vol 12, Iss 6, p 424 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2079-6374 06090060 |
| DOI: | 10.3390/bios12060424 |
| Popis: | Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed and robustness, require extensive infrastructure, and are neither affordable nor user-friendly. Thus, they have not been extensively applied in point-of-care diagnostics, particularly in low-resource settings. In this work, we have combined the loop-mediated isothermal amplification (LAMP) technology with a handheld portable device (SMART-LAMP) developed to perform real-time isothermal nucleic acid amplification reactions, based on simple colorimetric measurements, all of which are Bluetooth-controlled by a dedicated smartphone app. We have validated its diagnostic utility regarding different infectious diseases, including Schistosomiasis, Strongyloidiasis, and COVID-19, and analyzed clinical samples from suspected COVID-19 patients. Finally, we have proved that the combination of long-term stabilized LAMP master mixes, stored and transported at room temperature with our developed SMART-LAMP device, provides an improvement towards true point-of-care diagnosis of infectious diseases in settings with limited infrastructure. Our proposal could be easily adapted to the diagnosis of other infectious diseases. |
| Databáze: | Directory of Open Access Journals |
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