| Autor: |
Vincenzo A. Ellis, Victor Kalbskopf, Arif Ciloglu, Mélanie Duc, Xi Huang, Abdullah Inci, Staffan Bensch, Olof Hellgren, Vaidas Palinauskas |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Parasites & Vectors, Vol 15, Iss 1, Pp 1-12 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1756-3305 |
| DOI: |
10.1186/s13071-022-05373-w |
| Popis: |
Abstract Background Sequencing parasite genomes in the presence of host DNA is challenging. Sequence capture can overcome this problem by using RNA probes that hybridize with the parasite DNA and then are removed from solution, thus isolating the parasite DNA for efficient sequencing. Methods Here we describe a set of sequence capture probes designed to target 1035 genes (c. 2.5 Mbp) of the globally distributed avian haemosporidian parasite, Plasmodium relictum. Previous sequence capture studies of avian haemosporidians from the genus Haemoproteus have shown that sequencing success depends on parasitemia, with low-intensity, chronic infections (typical of most infected birds in the wild) often being difficult to sequence. We evaluate the relationship between parasitemia and sequencing success using birds experimentally infected with P. relictum and kept under laboratory conditions. Results We confirm the dependence of sequencing success on parasitemia. Sequencing success was low for birds with low levels of parasitemia ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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