Bacterial and fungal keratitis in Upper Egypt: In vitro screening of enzymes, toxins and antifungal activity

Autor: Abdullah A Gharamah, Ahmed M Moharram, Mady A Ismail, Ashraf K AL-Hussaini
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Blindness
cataract
eye lens
risk factors
treatment of cataract
Low vision care
multiple disabilities and visual impairment
reading performance
Accommodation
presbyopia
supraciliary segment implants
Amblyopia
citicoline
patching
Corneal endothelium
human corneal endothelial precursor cells
in vitro expansion
sphere forming assay
thermo-reversible gelation polymer
transportation
Endophthalmitis
explantation
fungal
intraocular lens
postoperative
recurrent
Colour perception
learning disabilities
school children
stereo acuity
testability
visual acuity
Contracted socket
dermis fat graft
porous orbital implant
reconstruction
Glucose
in vitro anticataract
lens
Moringa oliefera
′BARC I-125 Ocu-Prosta seeds′
choroidal melanoma
ciliary body melanoma
Episcleral plaque brachytherapy
retinal angioma
retinoblastoma
vasoproliferative tumor
Congenital glaucoma
gonioscopy
Retcam
Acoustic features
A-scan
B-scan
choroidal mass
ultrasound biomicroscopy
Cataract surgery
education
informed consent
multimedia
rural health
Lagophthalmos
silicone sling
temporalis muscle
Contrast sensitivity function
disability
function peak
multiple sclerosis
spatial frequency
Congenital cataract
developmental cataract
outcome
vision function
Bacterial
extracellular enzymes
keratitis
toxins
Ophthalmology
RE1-994
Zdroj: Indian Journal of Ophthalmology, Vol 62, Iss 2, Pp 196-203 (2014)
Druh dokumentu: article
ISSN: 0301-4738
1998-3689
DOI: 10.4103/0301-4738.116463
Popis: Purpose: This work was conducted to study the ability of bacterial and fungal isolates from keratitis cases in Upper Egypt to produce enzymes, toxins, and to test the isolated fungal species sensitivity to some therapeutic agents. Materials and Methods: One hundred and fifteen patients clinically diagnosed to have microbial keratitis were investigated. From these cases, 37 bacterial isolates and 25 fungal isolates were screened for their ability to produce extra-cellular enzymes in solid media. In addition, the ability of fungal isolates to produce mycotoxins and their sensitivity to 4 antifungal agents were tested. Results: Protease, lipase, hemolysins, urease, phosphatase, and catalase were detected respectively in 48.65%, 37.84%, 59.46%, 43.24%, 67.57%, and 100% out of 37 bacterial isolates tested. Out of 25 fungal isolates tested during the present study, 80% were positive for protease, 84% for lipase and urease, 28% for blood hemolysis, and 100% for phosphatase and catalase enzymes. Thirteen fungal isolates were able to produce detectable amounts of 7 mycotoxins in culture medium (aflatoxins (B1, B2, G1, and G2), sterigmatocystin, fumagillin, diacetoxyscirpenol, zearalenone, T-2 toxin, and trichodermin). Among the antifungal agents tested in this study, terbinafine showed the highest effect against most isolates in vitro. Conclusion: In conclusion, the ability of bacterial and fungal isolates to produce extracellular enzymes and toxins may be aid in the invasion and destruction of eye tissues, which, in turn, lead to vision loss.
Databáze: Directory of Open Access Journals
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