| Autor: |
Dong-Dong Chen, Ling Jiao, Yue Huang, Kang Xiao, Li-Ping Gao, Cao Chen, Qi Shi, Xiao-Ping Dong |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Aging Neuroscience, Vol 14 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1663-4365 |
| DOI: |
10.3389/fnagi.2022.898516 |
| Popis: |
The real-time quaking-induced conversion (RT-QuIC) assay has been developed and used as an in vitro diagnostic tool for Parkinson’s disease (PD). In this study, we established α-Syn RT-QuIC using recombinant human α-Syn as the substrate. All 5 brain homogenates of neuropathological PD cases and 13 skin homogenates of clinical PD cases showed positive results, whereas all the samples of negative controls remain negative. Meantime, randomly selected 6 skin samples of PD cases and 6 skin samples of sCJD cases showed negative in opposite prion RT-QuIC and α-Syn RT-QuIC. Our α-Syn RT-QuIC showed dose-dependent manner between the lag times and peak ThT fluorescent values. Additionally, the detecting limitation was about 10–7 dilution for brain tissues and 10–6 for skins. Those data indicate a reliable specificity and good sensitivity of the established α-Syn RT-QuIC in identifying and amplifying the misfolded α-Syn in brain and skin tissues of patients with PD. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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