The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
| Autor: | KATRIN ZIMMERMANN, MARLIS HOLTZ, LOC PHI-VAN |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Biological Research, Vol 40, Iss 2, Pp 185-192 (2007) |
| Druh dokumentu: | article |
| ISSN: | 0716-9760 0717-6287 |
| Popis: | We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase |
| Databáze: | Directory of Open Access Journals |
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