Comparative transcriptomics reveals PrrAB-mediated control of metabolic, respiration, energy-generating, and dormancy pathways in Mycobacterium smegmatis

Autor:	Jason D. Maarsingh, Shanshan Yang, Jin G. Park, Shelley E. Haydel
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Mycobacterium smegmatis Mycobacterium tuberculosis prrAB Two-component system RNA-seq Transcriptomics Biotechnology TP248.13-248.65 Genetics QH426-470
Zdroj:	BMC Genomics, Vol 20, Iss 1, Pp 1-16 (2019)
Druh dokumentu:	article
ISSN:	1471-2164
DOI:	10.1186/s12864-019-6105-3
Popis:	Abstract Background Mycobacterium smegmatis is a saprophytic bacterium frequently used as a genetic surrogate to study pathogenic Mycobacterium tuberculosis. The PrrAB two-component genetic regulatory system is essential in M. tuberculosis and represents an attractive therapeutic target. In this study, transcriptomic analysis (RNA-seq) of an M. smegmatis ΔprrAB mutant was used to define the PrrAB regulon and provide insights into the essential nature of PrrAB in M. tuberculosis. Results RNA-seq differential expression analysis of M. smegmatis wild-type (WT), ΔprrAB mutant, and complementation strains revealed that during in vitro exponential growth, PrrAB regulates 167 genes (q
Databáze:	Directory of Open Access Journals
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