The effect of feeding on CO2 production and energy expenditure in ponies measured by indirect calorimetry and the 13C-bicarbonate technique

Autor: R.B. Jensen, T.D. Kyrstein, P. Junghans, A.H. Tauson
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Animal, Vol 9, Iss 11, Pp 1778-1785 (2015)
Druh dokumentu: article
ISSN: 1751-7311
17517311
DOI: 10.1017/S1751731115001330
Popis: Energy expenditure (EE) can be estimated based on respiratory gas exchange measurements, traditionally done in respiration chambers by indirect calorimetry (IC). However, the 13C-bicarbonate technique (13C-BT) might be an alternative minimal invasive method for estimation of CO2 production and EE in the field. In this study, four Shetland ponies were used to explore the effect of feeding on CO2 production and EE measured simultaneously by IC and 13C-BT. The ponies were individually housed in respiration chambers and received either a single oral or intravenous (IV) bolus dose of 13C-labelled sodium bicarbonate (NaH13CO3). The ponies were fed haylage 3 h before (T−3), simultaneously with (T0) or 3 h after (T+3) administration of 13C-bicarbonate. The CO2 produced and O2 consumed by the ponies were measured for 6 h with both administration routes of 13C-bicarbonate at the three different feeding times. Feeding time affected the CO2 production (P
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