| Autor: |
Nadezhda B. Rudometova, Anastasia A. Fando, Lyubov A. Kisakova, Denis N. Kisakov, Mariya B. Borgoyakova, Victoria R. Litvinova, Vladimir A. Yakovlev, Elena V. Tigeeva, Danil I. Vahitov, Sergey V. Sharabrin, Dmitriy N. Shcherbakov, Veronika I. Evseenko, Ksenia I. Ivanova, Andrei S. Gudymo, Tatiana N. Ilyicheva, Vasiliy Yu. Marchenko, Alexander A. Ilyichev, Andrey P. Rudometov, Larisa I. Karpenko |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Vaccines, Vol 12, Iss 2, p 143 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2076-393X |
| DOI: |
10.3390/vaccines12020143 |
| Popis: |
In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus—the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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