| Autor: |
Mohammed Almalaysha, Arshdeep Singh, Sura A. Muhsin, Anna V. Carlson, Kate E. Trout, Amit Morey, Shuping Zhang, Lakshmikantha H. Channaiah, Mahmoud Almasri |
| Jazyk: |
angličtina |
| Rok vydání: |
2025 |
| Předmět: |
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| Zdroj: |
Sensors and Actuators Reports, Vol 9, Iss , Pp 100257- (2025) |
| Druh dokumentu: |
article |
| ISSN: |
2666-0539 |
| DOI: |
10.1016/j.snr.2024.100257 |
| Popis: |
This paper presents an investigation of a fluidic-based impedance biosensor for rapid and accurate detection of Salmonella Typhimurium in raw chicken carcass rinsate. The biosensor is engineered with multiple distinct regions that concentrates Salmonella antigens to a detectable level, subsequently trapping the concentrated Salmonella samples on top of the detection interdigitated electrode array coated with a specific Salmonella antibody, maximizing the number of captured antigens. Detection is achieved through the antibody-antigen binding process, where binding events changes impedance values, providing a reliable method for identifying and quantifying Salmonella. The biosensor demonstrated a low limit of detection (LOD) of 1–2 cells/ml within 40–50 min. The findings demonstrated that the biosensor distinguishes low concentrations of live Salmonella cells, even in the presence of high concentrations of dead Salmonella cells, and non-specific binding pathogens viz., Listeria monocytogenes and E. coli O157:H7. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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