Fast, automated, continuous energy scans for experimental phasing at the BioMAX beamline
| Autor: | Ishkhan Gorgisyan, Paul Bell, Michele Cascella, Mikel Eguiraun, Áureo Freitas, Julio Lidon-Simon, Jie Nan, Carla Takahashi, Hamed Tarawneh, Thomas Ursby, Ana Gonzalez |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Synchrotron Radiation, Vol 30, Iss 5, Pp 885-894 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1600-5775 16005775 |
| DOI: | 10.1107/S1600577523005738 |
| Popis: | In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously operational at BioMAX. The implementation of the continuous energy scan facilitates the prompt access to the anomalous scattering data, required for the SAD and MAD experiments. |
| Databáze: | Directory of Open Access Journals |
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