Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering

Autor: Agustin Enciso-Martinez, Edwin Van Der Pol, Chi M. Hau, Rienk Nieuwland, Ton G. Van Leeuwen, Leon W.M.M. Terstappen, Cees Otto
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Extracellular Vesicles, Vol 9, Iss 1 (2020)
Druh dokumentu: article
ISSN: 2001-3078
20013078
DOI: 10.1080/20013078.2020.1730134
Popis: Extracellular vesicles (EVs) present in blood originate from cells of different origins such as red blood cells (RBCs), platelets and leukocytes. In patients with cancer, a small portion of EVs originate from tumour cells and their load is associated with poor clinical outcome. Identification of these tumour-derived extracellular vesicles (tdEVs) is difficult as they are outnumbered by EVs of different tissue of origin as well a large number of lipoproteins (LPs) that are in the same size range. In order to detect tdEVs from the abundant presence of other particles, single-particle techniques are necessary. Here, synchronous Rayleigh and Raman scattering is used for that purpose. This combination of light scattering techniques identifies optically trapped single particles based on Rayleigh scattering and distinguishes differences in chemical composition of particle populations based on Raman scattering. Here, we show that tdEVs can be distinguished from RBC EVs and LPs in a label-free manner and directly in suspension.
Databáze: Directory of Open Access Journals
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