Structural and Expression Analysis of Uricase mRNA from Lotus japonicus
| Autor: | Ken-ichi Takane, Shigeyuki Tajima, Hiroshi Kouchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 13, Iss 10, Pp 1156-1160 (2000) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI.2000.13.10.1156 |
| Popis: | Uricase (nodulin-35) cDNA, LjUr, was isolated from nodules of a model legume, Lotus japonicus. LjUr expression was most abundant in nodules, although it was detected in nonsymbiotic tissues as well, particularly in roots. Expression in nodules was detected in uninfected cells, nodule parenchyma, and, more intensely, in vascular bundles. Phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide-transporting legumes form two distinct clades. LjUr is in the cluster of amide-transport legumes even though L. japonicus bears determinate nodules. |
| Databáze: | Directory of Open Access Journals |
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