| Autor: |
Nan Cai, Wenqi Luo, Lijun Yao, Xiuting Li, Zuzhe Wang, Hong Xu, Hui Li, Zhangli Hu, Weiyang Bao, Xu Xu |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 75, Iss , Pp 104229- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2020.104229 |
| Popis: |
Sea cucumbers are valuable marine foods as well as a source of traditional medicines with various bioactivities. Macrophage activation by small-molecule oligopeptides obtained from the sea cucumber Apostichopus japonicus (SCOP) were investigated. After SCOP were proven nontoxic by oral administration and CCK-8 assays, we measured immune-stimulatory mRNA, pro-inflammatory cytokines, and NO production levels and determined the molecular mechanism of macrophage stimulation. Our results demonstrated that SCOP upregulated both the mRNA levels and the secretion of NO, TNF-α, and IL-6 in a dose-dependent manner. More importantly, SCOP contributed to the stimulation of macrophages through the upregulation of the NF-κB and MAPK signaling pathways. Taken together, these results indicated that SCOP exerted macrophage activation effects on RAW264.7 cells and that they could potentially be used as a functional food supplement in treating immune suppression. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect