eQTL regulates OsHAK5 in enhancing potassium content under salt stress in rice

Autor: Akshara Balachandra1, Varanavasiappan Shanmugam1, Kumar K. Krish1, Ravichandran Veerasamy2, Kokiladevi Easwaran1, Kaleeswari Ramaiah Kutralingam3, Sudhakar Duraialagaraja1, Arul Loganathan1
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Electronic Journal of Plant Breeding, Vol 14, Iss 4, Pp 1345-1353 (2023)
Druh dokumentu: article
ISSN: 0975-928X
DOI: 10.37992/2023.1404.181
Popis: To alleviate salt stress in rice, the role of potassium in countering the ill effects of sodium is gaining importance. Towards this, OsHAK5 a member of the high-affinity potassium transporter family was known for enhancing the intracellular potassium under reduced/low K+ availability during salt stress. The full-length cDNA of OsHAK5 was cloned and sequenced from the roots of Pokkali and cv. IR64 however, no nucleotide polymorphism detected. Interestingly, the expression of OsHAK5 in roots of salt-tolerant landrace Pokkali and salt susceptible cv. IR64 revealed a significant upregulation in the former under low K+ (1/4th) condition. Subsequent analysis of the amino acid sequence of CBL-interacting protein kinase (OsCIPK23), the upstream regulator of OsHAK5 revealed two INDELs and one of them turned out to be functionally relevant. The OsCIPK23 variant from salt-tolerant Pokkali should be favorably regulating the expression of the downstream OsHAK5 leading to an increased K+ uptake under salt stress.
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