Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm

Autor:	Valentina Longobardi, Giovanni della Valle, Francesco Iannaccone, Alfonso Calabria, Gabriele Di Vuolo, Sara Damiano, Roberto Ciarcia, Bianca Gasparrini
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	buffalo sperm crocin oxidative stress Animal culture SF1-1100
Zdroj:	Italian Journal of Animal Science, Vol 20, Iss 1, Pp 2095-2101 (2021)
Druh dokumentu:	article
ISSN:	1594-4077 1828-051X 1828051X
DOI:	10.1080/1828051X.2021.1997653
Popis:	This work aimed to evaluate the effect of crocin on frozen-thawed sperm quality in buffalo. Spermatozoa were incubated in Tyrode’s Albumin Lactate Pyruvate medium supplemented with 0, 0.5, 1, and 2 mM crocin for 2 h. Sperm motility was evaluated by phase-contrast microscopy, viability and acrosome integrity by Trypan blue Giemsa staining, and membrane functional integrity by the hypoosmotic swelling test. The DNA fragmentation was evaluated by Tunel and ROS levels by spectrofluorometric analysis. The treatment with 2 mM of crocin increased (P
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/1c759694df9b4c399c217ff21e4f38da Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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